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Continuum Digital Assay Platform

The advent of digital PCR has unlocked new levels of precision and sensitivity for nucleic acid quantification, but high costs and complex workflows have put this technology out of reach for many research and clinical applications.  Continuum, the digital assay platform under development at Dropworks, represents a new generation in assay technology, providing the sensitivity and accuracy of digital PCR within a simple, one-step workflow at similar costs to real-time PCR.  Continuum puts the power of digital PCR into the hands of every researcher. Key features include:

  • Droplets are immediately processed upon generation and undergo nearly instantaneous heating and cooling, making amplification more uniform and data interpretation clearer

  • All of the steps of digital PCR are contained in a single instrument, giving a familiar real-time PCR workflow, reducing cost, and minimizing droplet loss

  • Proprietary chemistry prevents cross-contamination and enables a common flow pathway, eliminating costly consumables and fluid handling steps associated with other digital PCR approaches

  • Intuitive software makes quantification easy and straightforward, and data can be extracted to text file or SQL-based LIMS

 

Why Continuum? By offering the power of digital PCR with the cost and simplicity of real-time PCR, the better question is, why not?

 

Continuum: Digital PCR for Everyone™ (Coming Soon)

Continuum Platform Benefits

High Sensitivity
and Data Quality​

Full digital PCR sensitivity (>30,000 partitions) and absolute quantification without standard curves or technical replicates.  

High Throughput with Rapid Turnaround Time

Each sample requires 20-30 minutes to get an answer, and because the system can process multiple single-file samples at once, an entire 96-well plate can be processed in under 4 hours.

Simple, Single-step Workflow

Load your 96-well plate, perform a simple 5-minute setup using the intuitive software, hit run, and walk away.

Four Channel Multiplexing

Our advanced optics allow for up to four optical channels to be quantified simultaneously per well, increasing throughput and enabling complex gene expression and cancer biomarker assays.

Low Cost

Acquisition costs are similar to mid-range qPCR instruments, and operating costs for the system per sample are on par with qPCR at any scale, given the reduced need for technical replicates and standard curves.

Small Footprint

Integration of partitioning, thermal cycling, and signal measurement into a single instrument gives a compact footprint not much larger than a laptop, saving critical bench space.

Specifications Table

Cy™ is a trademark of GE Healthcare. FAM™, JOE™, and ROX™ are trademarks of Life Technologies Inc.

For Research Use Only. Not for use in diagnostic procedures.

Performance Highlights

Uniform and stable droplets enable clear separation of positive and negative data points

Continuum provides the full power of digital PCR, measuring over 30,000 partitions per sample to give highly precise and sensitive results. On-demand droplet formation and optimized chemistry improve specificity of target amplification, making it simpler to distinguish between positive and negative partitions.

All-in-one instrument eliminates cross-contamination

Our self-contained instrument and surface-stabilized partitions eliminate cross-contamination between samples and introduced when transferring between digital PCR steps, proven by consistent, clean no-template controls that give you confidence in the integrity of your data.

Strong multiplexing capabilities

Continuum has the ability to measure fluorescent signals in up to 4 different optical channels for each sample, and it can accept both intercalating dyes and hydrolysis probes, either separately or simultaneously.  On-demand droplet generation and uniform processing give distinct amplification, making cluster identification in our software straightforward, as can be seen in the assay for two different regions of the CMV genome (left).

Instantaneous and uniform picoliter heating improves repeatability in complicated SNP assays

Competing primer pairs in SNP assays can make achieving reliable clustering in digital PCR difficult, obscuring results.  The unique nature of our continuous flow process ensures each partition achieves target temperature nearly instantaneously, making assay tuning simpler and more reliable.  To the right, rare SNV genes in a high wild-type background for an EGFR lung cancer assay show clean clustering, even for partitions with an occupancy greater than one.